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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (401)
发育生物学 (289)
分子生物学 (1014)
干细胞 (219)
免疫学 (564)
神经科学 (612)
生物化学 (1256)
生物物理学 (122)
微生物学 (1061)
系统生物学 (281)
细胞生物学 (1890)
植物科学 (982)
Bio-protocol 期刊
特刊
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
Protocols shared by authors via RaP service
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IFNγ ELISpot
Author:
Christopher M. Haqq
Date: May 27, 2021,Views:972
Original protocol used in research article:
A lymph node\–targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2
Science Advances, Feb 4, 2021; DOI:
10.1126/sciadv.abe5819
Single hepatocyte collection
Authors:
S. Sun
and
J. Vijg
Date: May 26, 2021,Views:859
Original protocol used in research article:
Single-cell analysis reveals different age-related somatic mutation profiles between stem and differentiated cells in human liver
Science Advances, Jan 30, 2020; DOI:
10.1126/sciadv.aax2659
Quantification of food intake
Authors:
Caroline Lei Wee
,
Sam Kunes
and
Erin Song
Date: May 26, 2021,Views:1029
Original protocol used in research article:
A bidirectional network for appetite control in larval zebrafish
eLife, Oct 18, 2019; DOI:
10.7554/eLife.43775
Whole-mount immunostaining
Authors:
Caroline Lei Wee
,
Sam Kunes
and
Erin Song
Date: May 26, 2021,Views:1030
Original protocol used in research article:
A bidirectional network for appetite control in larval zebrafish
eLife, Oct 18, 2019; DOI:
10.7554/eLife.43775
Fecundity assays
Authors:
Brooke Zanco
and
Matthew DW Piper
Date: May 26, 2021,Views:1587
Original protocol used in research article:
A dietary sterol trade-off determines lifespan responses to dietary restriction in
Drosophila melanogaster
females
eLife, Jan 26, 2021; DOI:
10.7554/eLife.62335
scRNA/TCR-seq data processing
Authors:
Paul Gueguen
,
Joshua J. Waterfall
and
Sebastian Amigorena
Date: May 26, 2021,Views:952
Original protocol used in research article:
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer
Science Immunology, Jan 29, 2021; DOI:
10.1126/sciimmunol.abd5778
Generation of cell lines stably expressing ACVR1-IPF
Authors:
Anassuya Ramachandran
and
Caroline S Hill
Date: May 25, 2021,Views:798
Original protocol used in research article:
TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI:
10.7554/eLife.31756
Flow cytometry analysis of the CAR-transduced cells
Authors:
Vanaja Konduri
and
William K. Decker
Date: May 25, 2021,Views:945
Original protocol used in research article:
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI:
10.1126/scitranslmed.abc3196
Preparation of DCs
Authors:
Emilie V Russler-Germain
and
Chyi-Song Hsieh
Date: May 25, 2021,Views:934
Original protocol used in research article:
Gut Helicobacter presentation by multiple dendritic cell subsets enables context-specific regulatory T cell generation
eLife, Feb 3, 2021; DOI:
10.7554/eLife.54792
Immunocytochemistry
Authors:
Y. Zheng
,
G. Zhao
and
J. Fu
Date: May 24, 2021,Views:831
Original protocol used in research article:
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI:
10.1126/sciadv.aax5933
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