Bio-101 - Improve Research Reproducibility

co-immunoprecipitation and immunoblotting

Authors: Taifeng Zhou, Peiqiang Su and Yingzi Yang
Date: May 12, 2021，Views：2401

Original protocol used in research article：

Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin
eLife, Mar 18, 2020; DOI: 10.7554/eLife.52779

T cell isolation

Authors: Scott B Thompson and Jordan Jacobelli
Date: May 11, 2021，Views：1108

Original protocol used in research article：

Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046

Barley spikelet morphometry analysis

Author: Ravi Koppolu
Date: May 11, 2021，Views：851

Original protocol used in research article：

Transcriptional landscapes of floral meristems in barley
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abf0832

Culture of mouse/rat primary neurons

Authors: Richard L Huganir and Huaqiang Fang
Date: May 11, 2021，Views：1118

Original protocol used in research article：

An optimized CRISPR/Cas9 approach for precise genome editing in neurons
eLife, Mar 10, 2021; DOI: 10.7554/eLife.65202

Expression and purification of recombinant FOXO3-DBD

Authors: Judith Hagenbuchner, Tomas Obsil, Michael J Ausserlechner and Veronika Obsilova
Date: May 10, 2021，Views：789

Original protocol used in research article：

Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876

Binning approach and normalization

Authors: Nina Kirstein, Olivier Hyrien, Benjamin Audit and Aloys Schepers
Date: May 10, 2021，Views：862

Original protocol used in research article：

Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones
eLife, Mar 8, 2021; DOI: 10.7554/eLife.62161

Protein extraction and immunobloting

Authors: Ismael Lamas-Toranzo, Julieta G Hamze, María Jiménez-Movilla and Pablo Bermejo-Álvarez
Date: May 10, 2021，Views：894

Original protocol used in research article：

TMEM95 is a sperm membrane protein essential for mammalian fertilization
eLife, Jun 2, 2020; DOI: 10.7554/eLife.53913

Immunoblotting

Authors: Benjamin Demarco, Kaiwen Chen and Petr Broz
Date: May 10, 2021，Views：823

Original protocol used in research article：

Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc3465

Gene-specific m6A qPCR

Authors: Chris Bass and Youjun Zhang
Date: May 9, 2021，Views：1188

Original protocol used in research article：

Epitranscriptomic regulation of insecticide resistance
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe5903

Statistical analysis

Authors: David Chen, Josephine Wu and Gordana Vunjak-Novakovic
Date: May 9, 2021，Views：861

Original protocol used in research article：

Tissue engineered autologous cartilage-bone grafts for temporomandibular joint regeneration
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.abb6683

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