Bio-101 - Improve Research Reproducibility

Intestinal organoid cultures

Authors: Zuri Sullivan and Ruslan Medzhitov
Date: May 18, 2021，Views：855

Original protocol used in research article：

γδ T cells regulate the intestinal response to nutrient sensing
Science, Mar 19, 2021; DOI: 10.1126/science.aba8310

Data filtering and genome assembly

Authors: Reuben W Nowell and Timothy G Barraclough
Date: May 18, 2021，Views：823

Original protocol used in research article：

Evolutionary dynamics of transposable elements in bdelloid rotifers
eLife, Feb 5, 2021; DOI: 10.7554/eLife.63194

Data

Authors: Li Wang and Ni Huang
Date: May 17, 2021，Views：932

Original protocol used in research article：

Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508

Parameter optimizations of the three machine learning algorithm models

Authors: Ni Huang and Li Wang
Date: May 17, 2021，Views：849

Original protocol used in research article：

Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508

Whole Mount in situ Hybridization (WISH) on mouse embryos

Authors: Matthew J Anderson and Mark Lewandoski
Date: May 17, 2021，Views：829

Original protocol used in research article：

Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608

Characterization of CAR-transduced T cell cytotoxic potential

Authors: Vanaja Konduri and William K. Decker
Date: May 17, 2021，Views：862

Original protocol used in research article：

A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196

Immunofluorescence, Sirius red staining, and intensity quantitations

Authors: Denise Gay, Paul-Henri Romeo and Denise Gay
Date: May 16, 2021，Views：890

Original protocol used in research article：

Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aay3704

T cell migration assays

Author: Dong Wang
Date: May 16, 2021，Views：951

Original protocol used in research article：

Tumor immunological phenotype signature-based high-throughput screening for the discovery of combination immunotherapy compounds
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abd7851

Identification of gene loss

Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：898

Original protocol used in research article：

A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459

Gene family expansion and contraction

Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：923

Original protocol used in research article：

A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459

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