Bio-101 - Improve Research Reproducibility

Preparation of Aβ solution

Author: Peter Niraj Nirmalraj
Date: Jun 7, 2021，Views：869

Original protocol used in research article：

Complete aggregation pathway of amyloid β (1-40) and (1-42) resolved on an atomically clean interface
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz6014

Reverse transcription (RT) and quantitative RT-PCR analysis

Authors: Ziling Fang, Shelya X Zeng, Jianping Xiong and Hua Lu
Date: Jun 6, 2021，Views：834

Original protocol used in research article：

SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer
eLife, Mar 16, 2018; DOI: 10.7554/eLife.31275

Parallel reaction monitoring MS

Authors: Eric Chiles, Amanda Williams, Debashish Bhattacharya and Xiaoyang Su
Date: Jun 4, 2021，Views：868

Original protocol used in research article：

Metabolomic shifts associated with heat stress in coral holobionts
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd4210

Wounding Zebrafish Larval Epidermis by Laceration

Authors: Andrew S Kennard, Ellen Labuz, Christopher K Prinz and Julie A Theriot
Date: Jun 3, 2021，Views：1122

Original protocol used in research article：

Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis
eLife, Nov 23, 2020; DOI: 10.7554/eLife.62386

Real-Time PCR

Authors: Ming Xu and James L Kirkland
Date: Jun 3, 2021，Views：965

Original protocol used in research article：

Targeting senescent cells enhances adipogenesis and metabolic function in old age
eLife, Dec 19, 2015; DOI: 10.7554/eLife.12997

Reagents and antibodies

Author: Hongyu Deng
Date: Jun 3, 2021，Views：821

Original protocol used in research article：

PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion
Science Advances, Nov 19, 2020; DOI: 10.1126/sciadv.abd0276

Histone modification ChIP-seq on Arabidopsis thaliana plantlets

Authors: Juan Sebastian Ramirez Prado, David Latrasse and Moussa Benhamed
Date: Jun 3, 2021，Views：1215

Original protocol used in research article：

A new role for histone demethylases in the maintenance of plant genome integrity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.58533

Mice

Author: Bin Zhou
Date: Jun 3, 2021，Views：868

Original protocol used in research article：

Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair
Science, Feb 26, 2021; DOI: 10.1126/science.abc4346

Culturing splenic, hepatic and alveolar macrophages

Author: Alexandre A Steiner
Date: Jun 2, 2021，Views：822

Original protocol used in research article：

A leukotriene-dependent spleen-liver axis drives TNF production in systemic inflammation
Science Signaling, Apr 20, 2021; DOI: 10.1126/scisignal.abb0969

Study design

Authors: Adam Yala and Regina Barzilay
Date: Jun 1, 2021，Views：905

Original protocol used in research article：

Toward robust mammography-based models for breast cancer risk
Science Translational Medicine, Jan 27, 2021; DOI: 10.1126/scitranslmed.aba4373

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