Bio-101 - Improve Research Reproducibility

Trapping of photocycle intermediates

Authors: Orawan Jakdetchai, Johanna Becker-Baldus, Josef Wachtveitl and Clemens Glaubitz
Date: Apr 26, 2021，Views：840

Original protocol used in research article：

Probing the photointermediates of light-driven sodium ion pump KR2 by DNP-enhanced solid-state NMR
Science Advances, Mar 12, 2021; DOI: 10.1126/sciadv.abf4213

Cell preparation and scRNA-seq

Authors: Yiran Hou, Guoyan Zhao and Ting Wang
Date: Apr 25, 2021，Views：980

Original protocol used in research article：

Cellular diversity of the regenerating caudal fin
Science Advances, Aug 12, 2020; DOI: 10.1126/sciadv.aba2084

Quantification of ER ATP and Ca2+ levels by fluorescence ratiometry analysis in live cells

Author: Jing Yong
Date: Apr 23, 2021，Views：879

Original protocol used in research article：

Mitochondria supply ATP to the ER through a mechanism antagonized by cytosolic Ca²⁺
eLife, Sep 9, 2019; DOI: 10.7554/eLife.49682

ER and mitochondrial ATP level analysis by flow cytometry

Author: Jing Yong
Date: Apr 23, 2021，Views：1160

Original protocol used in research article：

Mitochondria supply ATP to the ER through a mechanism antagonized by cytosolic Ca²⁺
eLife, Sep 9, 2019; DOI: 10.7554/eLife.49682

Multivariate analyses of shape

Authors: V. B. Baliga and D. L. Altshuler
Date: Apr 23, 2021，Views：869

Original protocol used in research article：

Range of motion in the avian wing is strongly associated with flight behavior and body mass
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6670

RNAscope in situ hybridization

Authors: Alexander T Chesler and Claire E Le Pichon
Date: Apr 23, 2021，Views：862

Original protocol used in research article：

Dual leucine zipper kinase is required for mechanical allodynia and microgliosis after nerve injury
eLife, Jul 3, 2018; DOI: 10.7554/eLife.33910

Characterizing the pressure sensor

Authors: Yoonseok Park and John A. Rogers
Date: Apr 23, 2021，Views：931

Original protocol used in research article：

Wireless, skin-interfaced sensors for compression therapy
Science Advances, Dec 4, 2020; DOI: 10.1126/sciadv.abe1655

Immunohistochemistry and synaptic puncta analysis

Authors: W Christopher Risher and Cagla Eroglu
Date: Apr 23, 2021，Views：924

Original protocol used in research article：

Astrocytes refine cortical connectivity at dendritic spines
eLife, Dec 17, 2014; DOI: 10.7554/eLife.04047

Astrocyte primary cultures

Authors: Álvaro Herrero-Navarro and Guillermina López-Bendito
Date: Apr 22, 2021，Views：857

Original protocol used in research article：

Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming
Science Advances, Apr 7, 2021; DOI: 10.1126/sciadv.abe8978

Liposome dye release assay

Authors: Gemma E Swinscoe and Stephen Griffin
Date: Apr 21, 2021，Views：960

Original protocol used in research article：

Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy
eLife, Nov 10, 2020; DOI: 10.7554/eLife.52555

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