Bio-101 - Improve Research Reproducibility

Biotinylation of Smc3p-AviTag reporters

Authors: Siheng Xiang and Douglas Koshland
Date: Apr 21, 2021，Views：891

Original protocol used in research article：

Cohesin architecture and clustering in vivo
eLife, Feb 17, 2021; DOI: 10.7554/eLife.62243

Chromatin Immunoprecipitation, sequencing and data analysis in Drosophila S2 cells

Authors: Anand K Singh, Jie Zhang, Daniel Hebenstreit and Saverio Brogna
Date: Apr 20, 2021，Views：1442

Original protocol used in research article：

Evidence of slightly increased Pol II pausing in UPF1-depleted Drosophila melanogaster cells
microPublication Biology, Oct 16, 2020; DOI: 10.17912/micropub.biology.000319

Single-nucleus ATAC-seq data generation

Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 19, 2021，Views：999

Original protocol used in research article：

Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522

LNP quantification and encapsulation

Author: Dan Peer
Date: Apr 19, 2021，Views：1064

Original protocol used in research article：

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450

Flow cytometric analysis (FACS)

Authors: Andy Peng Xiang and Zhengqi Lu
Date: Apr 18, 2021，Views：806

Original protocol used in research article：

Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating ROR\γt
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abe4827

Purification of cells by FACS

Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：821

Original protocol used in research article：

Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328

RNA-seq

Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：840

Original protocol used in research article：

Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328

Expression, purification and on-bead reconstitution of MsbA and MscS into peptidiscs

Authors: Gabriella Angiulli, Franck Duong Van Hoa and Thomas Walz
Date: Apr 16, 2021，Views：981

Original protocol used in research article：

New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
eLife, Mar 3, 2020; DOI: 10.7554/eLife.53530

Open-field test

Authors: Byung Tae Choi and Da Hee Jung
Date: Apr 16, 2021，Views：1682

Original protocol used in research article：

Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359

Chronic mild stress

Authors: Huafu Chen and Zili You
Date: Apr 15, 2021，Views：924

Original protocol used in research article：

IL4-driven microglia modulate stress resilience through BDNF-dependent neurogenesis
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abb9888

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