Bio-101 - Improve Research Reproducibility

RNA-seq and differential expression power analysis

Author: Benjamin Jung Fair
Date: Apr 6, 2021，Views：976

Original protocol used in research article：

Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929

Confocal microscopy, live cell imaging, and intravital microscopy

Authors: Sang Hun Lee and David L. Sacks
Date: Apr 5, 2021，Views：913

Original protocol used in research article：

M2-like dermal macrophages are maintained via IL-4/CCL24–mediated cooperative interaction with eosinophils in cutaneous leishmaniasis
Science Immunology, Apr 9, 2020; DOI: 10.1126/sciimmunol.aaz4415

Oviductal sperm counting

Authors: Wipawee Winuthayanon and Carmen J Williams
Date: Apr 2, 2021，Views：787

Original protocol used in research article：

Oviductal estrogen receptor α signaling prevents protease-mediated embryo death
eLife, Dec 1, 2015; DOI: 10.7554/eLife.10453

Immunofluorescence

Authors: Shang Yin Chiang, Ruey-Hwa Chen and Shen-Ju Chou
Date: Apr 2, 2021，Views：830

Original protocol used in research article：

Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abc6093

Cell staining and imaging

Author: Anne E Carpenter
Date: Apr 2, 2021，Views：920

Original protocol used in research article：

Systematic morphological profiling of human gene and allele function via Cell Painting
eLife, Mar 18, 2017; DOI: 10.7554/eLife.24060

NMR measurements of phase-separating samples

Authors: Rob W. H. Ruigrok and Martin Blackledge
Date: Apr 2, 2021，Views：859

Original protocol used in research article：

Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly
Science Advances, Apr 1, 2020; DOI: 10.1126/sciadv.aaz7095

Calculation of dice scores

Authors: Yunyun Han and Peng Fei
Date: Apr 1, 2021，Views：827

Original protocol used in research article：

Bi-channel image registration and deep-learning segmentation (BIRDS) for efficient, versatile 3D mapping of mouse brain
eLife, Jan 18, 2021; DOI: 10.7554/eLife.63455

Differentiation of human and mouse THGM cells in vitro

Authors: Javad Rasouli, Bogoljub Ciric and Abdolmohamad Rostami
Date: Apr 1, 2021，Views：916

Original protocol used in research article：

A distinct GM-CSF+ T helper cell subset requires T-bet to adopt a TH1 phenotype and promote neuroinflammation
Science Immunology, Oct 22, 2020; DOI: 10.1126/sciimmunol.aba9953

PDC isolation and culture

Authors: Li-Wei Dong, Ye-Xiong Tan and Hong-Yang Wang
Date: Apr 1, 2021，Views：822

Original protocol used in research article：

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aay0152

Activity recording

Authors: Yusaku Hontani, Fei Xia and Chris Xu
Date: Mar 31, 2021，Views：959

Original protocol used in research article：

Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abf3531

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