Bio-101 - Improve Research Reproducibility

Optogenetic control of CA production

Authors: Meng C Wang and Jeffrey J Tabor
Date: Mar 31, 2021，Views：843

Original protocol used in research article：

Optogenetic control of gut bacterial metabolism to promote longevity
eLife, Dec 16, 2020; DOI: 10.7554/eLife.56849

Preparation of αSyn PFFs

Author: Jin-Song Bian
Date: Mar 30, 2021，Views：878

Original protocol used in research article：

Anti\–Na+/K+-ATPase immunotherapy ameliorates \α-synuclein pathology through activation of Na+/K+-ATPase \α1\–dependent autophagy
Science Advances, Jan 26, 2021; DOI: 10.1126/sciadv.abc5062

ND-labeling C. elegans worms

Authors: Masazumi Fujiwara, Eriko Kage-Nakadai and Simo Sun
Date: Mar 30, 2021，Views：783

Original protocol used in research article：

Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI: 10.1126/sciadv.aba9636

Zebrafish retinal dissociation, cell sorting, and methanol fixation

Authors: Patrick Boyd and David R. Hyde
Date: Mar 29, 2021，Views：902

Original protocol used in research article：

Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598

Cortical excitatory neuron differentiation

Author: Dina Simkin
Date: Mar 29, 2021，Views：1002

Original protocol used in research article：

Dyshomeostatic modulation of Ca²⁺-activated K⁺ channels in a human neuronal model of KCNQ2 encephalopathy
eLife, Feb 5, 2021; DOI: 10.7554/eLife.64434

Parasite strains, culturing and resistance selection

Author: Eva S Istvan
Date: Mar 28, 2021，Views：881

Original protocol used in research article：

Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis
eLife, Mar 19, 2019; DOI: 10.7554/eLife.40529

PIV analysis

Author: HaiYang Wang
Date: Mar 28, 2021，Views：969

Original protocol used in research article：

Symmetry breaking in hydrodynamic forces drives meiotic spindle rotation in mammalian oocytes
Science Advances, Apr 3, 2020; DOI: 10.1126/sciadv.aaz5004

Spinning-disc confocal and 2P3A-DSLM imaging

Authors: Jia Zhao, Yanmei Liu and Liangyi Chen
Date: Mar 27, 2021，Views：1114

Original protocol used in research article：

In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development
eLife, Jan 29, 2019; DOI: 10.7554/eLife.41540

Phylogenetic analysis of sleep-relevant genes

Authors: Junko Kusumi, Hiroyuki J. Kanaya and Taichi Q. Itoh
Date: Mar 27, 2021，Views：797

Original protocol used in research article：

A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415

Ce3D+ and Ce3D++ Tissue Clearing

Authors: Matthew J. Anderson, Valentin Magidson and Mark Lewandoski
Date: Mar 26, 2021，Views：1226

Original protocol used in research article：

Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608

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