Protocols shared by authors via RaP service
Journal
Bone sectioning, immunostaining, and confocal imaging
Authors: Lei Ding and Sean J Morrison
Date: Apr 9, 2021,Views:862
Original protocol used in research article:
Hematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1
eLife, Mar 30, 2015; DOI: 10.7554/eLife.05521
Statistical analysis
Authors: Thanaphong Phongpreecha and Thomas J. Montine
Date: Apr 8, 2021,Views:829
Original protocol used in research article:
Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575
Contribution of inter-relatedness chimpanzees to differential expression analysis
Author: Benjamin Jung Fair
Date: Apr 8, 2021,Views:806
Original protocol used in research article:
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
Single-nucleus RNA-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 7, 2021,Views:1007
Original protocol used in research article:
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
Microtubule co-sedimentation from worm extract
Author: Shaohe Wang
Date: Apr 7, 2021,Views:821
Original protocol used in research article:
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Extending 3' UTRs in the transcriptome index
Authors: Lauren M Saunders, Cole Trapnell and David M Parichy
Date: Apr 7, 2021,Views:975
Original protocol used in research article:
Thyroid hormone regulates distinct paths to maturation in pigment cell lineages
eLife, May 29, 2019; DOI: 10.7554/eLife.45181
Cell transformation assay
Authors: Karoliina Stefanius and Kim Orth
Date: Apr 7, 2021,Views:947
Original protocol used in research article:
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
In situ hybridization
Authors: Kaiwen Kam and Carolina Thörn Perez
Date: Apr 7, 2021,Views:878
Original protocol used in research article:
Opioids modulate an emergent rhythmogenic process to depress breathing
eLife, Dec 16, 2019; DOI: 10.7554/eLife.50613
Luciferase assay
Authors: Lihui Han and dapeng ma
Date: Apr 7, 2021,Views:855
Original protocol used in research article:
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response
Science Advances, Mar 24, 2021; DOI: 10.1126/sciadv.abc1834
IP and immunoblot analysis
Authors: Lihui Han and Dapeng Ma
Date: Apr 7, 2021,Views:860
Original protocol used in research article:
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response
Science Advances, Mar 24, 2021; DOI: 10.1126/sciadv.abc1834