Bio-101 - Improve Research Reproducibility

Sperm sample collection and sRNA sequencing

Authors: Yanbo Wang and Xi Chen
Date: Mar 20, 2021，Views：870

Original protocol used in research article：

Sperm microRNAs confer depression susceptibility to offspring
Science Advances, Feb 9, 2021; DOI: 10.1126/sciadv.abd7605

HPTS staining and confocal imaging

Author: Rui Miao
Date: Mar 19, 2021，Views：1171

Original protocol used in research article：

Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abd4113

Golgi impregnations

Author: Nicholas James Strausfeld
Date: Mar 19, 2021，Views：863

Original protocol used in research article：

An insect-like mushroom body in a crustacean brain
eLife, Sep 26, 2017; DOI: 10.7554/eLife.29889

Analysis of protein stability by synthesis shutoff

Authors: Ira Buntenbroich, Tânia Simões and Mafalda Escobar-Henriques
Date: Mar 19, 2021，Views：1123

Original protocol used in research article：

Cdc48 regulates a deubiquitylase cascade critical for mitochondrial fusion
eLife, Jan 8, 2018; DOI: 10.7554/eLife.30015

Cell culturing and treatments

Author: Massimiliano Clamer
Date: Mar 19, 2021，Views：856

Original protocol used in research article：

One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife, Feb 17, 2021; DOI: 10.7554/eLife.59303

Protein extraction from sucrose gradient fractions

Author: Massimiliano Clamer
Date: Mar 19, 2021，Views：935

Original protocol used in research article：

One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife, Feb 17, 2021; DOI: 10.7554/eLife.59303

Bacterial isolation, genome amplification and DNA extraction

Authors: Panagiotis Sapountzis and Panagiotis Sapountzis
Date: Mar 19, 2021，Views：887

Original protocol used in research article：

Reconstructing the functions of endosymbiotic Mollicutes in fungus-growing ants
eLife, Nov 20, 2018; DOI: 10.7554/eLife.39209

Cryo-EM data collection

Authors: Sunbin Deng and Ronen Marmorstein
Date: Mar 18, 2021，Views：788

Original protocol used in research article：

Molecular basis for N-terminal alpha-synuclein acetylation by human NatB
eLife, Sep 4, 2020; DOI: 10.7554/eLife.57491

PIV

Authors: James F Pelletier and Timothy J Mitchison
Date: Mar 18, 2021，Views：949

Original protocol used in research article：

Co-movement of astral microtubules, organelles and F-actin by dynein and actomyosin forces in frog egg cytoplasm
eLife, Dec 7, 2020; DOI: 10.7554/eLife.60047

Characterization techniques and analyses

Author: Hadi Shafiee
Date: Mar 18, 2021，Views：852

Original protocol used in research article：

Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354

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