Bio-101 - Improve Research Reproducibility

Reduced silver staining

Authors: Gabriella Hannah Wolff and Nicholas James Strausfeld
Date: Mar 18, 2021，Views：842

Original protocol used in research article：

An insect-like mushroom body in a crustacean brain
eLife, Sep 26, 2017; DOI: 10.7554/eLife.29889

Cell Clustering and cluster identification

Author: Michael L Wallace
Date: Mar 18, 2021，Views：840

Original protocol used in research article：

Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271

First and second strand cDNA synthesis and pre-amplification

Author: Arthur M Talman
Date: Mar 18, 2021，Views：884

Original protocol used in research article：

Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33105

Generation of transgenic lines

Authors: Oriol Llorà-Batlle and Alfred Cortés
Date: Mar 17, 2021，Views：757

Original protocol used in research article：

Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz5057

Isolation of T-cell subpopulations

Authors: Anastasia A Minervina and Mikhail V Pogorelyy
Date: Mar 17, 2021，Views：1347

Original protocol used in research article：

Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
eLife, Feb 21, 2020; DOI: 10.7554/eLife.53704

Strand-specific lncRNA sequencing and analysis

Authors: Shiqi Luo and Xin Zhou
Date: Mar 17, 2021，Views：874

Original protocol used in research article：

Gene reuse facilitates rapid radiation and independent adaptation to diverse habitats in the Asian honeybee
Science Advances, Dec 17, 2020; DOI: 10.1126/sciadv.abd3590

Generate dot surface of the channel hole for CALHM1 using HOLE program

Authors: Xue Yang and Yuequan Shen
Date: Mar 16, 2021，Views：906

Original protocol used in research article：

Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161

MS/MS biclustering and molecular networking

Author: Dapeng Li
Date: Mar 16, 2021，Views：745

Original protocol used in research article：

Information theory tests critical predictions of plant defense theory for specialized metabolism
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz0381

Protein expression and purification

Author: Yue Ren
Date: Mar 16, 2021，Views：923

Original protocol used in research article：

Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161

scVelo

Author: Bryan Marsh
Date: Mar 15, 2021，Views：2174

Original protocol used in research article：

Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266

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