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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (420)
发育生物学 (311)
分子生物学 (1101)
干细胞 (243)
免疫学 (584)
神经科学 (676)
生物化学 (1346)
生物物理学 (156)
微生物学 (1134)
系统生物学 (322)
细胞生物学 (2063)
植物科学 (1036)
Bio-protocol 期刊
Bio-101 实验手册
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
Protocols shared by authors via RaP service
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Subcellular fractionation
Authors:
Tyler B Faust
and
Alan D Frankel
Date: Feb 22, 2021,Views:854
Original protocol used in research article:
The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI:
10.7554/eLife.31879
Sequences, plasmids, transfection and RNA interference
Author:
Zhou Yu
Date: Feb 22, 2021,Views:829
Original protocol used in research article:
Lys29-linkage of ASK1 by Skp1−Cullin 1−Fbxo21 ubiquitin ligase complex is required for antiviral innate response
eLife, Apr 11, 2016; DOI:
10.7554/eLife.14087
Immune signature, immune infiltration, and nontumor fraction
Authors:
Yue Wang
and
Da Yang
Date: Feb 22, 2021,Views:828
Original protocol used in research article:
LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance
Science Advances, Feb 9, 2021; DOI:
10.1126/sciadv.abb3555
Bisulfite sequencing and TAB sequencing
Authors:
Gerd P. Pfeifer
and
Zhijun Huang
Date: Feb 22, 2021,Views:839
Original protocol used in research article:
The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins
Science Advances, Jan 20, 2021; DOI:
10.1126/sciadv.abb9149
Synthesis of FX and preparation of FX@HP
Authors:
Minjie Sun
and
Zhaoting Li
Date: Feb 22, 2021,Views:848
Original protocol used in research article:
Targeting pulmonary tumor microenvironment with CXCR4-inhibiting nanocomplex to enhance anti–PD-L1 immunotherapy
Science Advances, May 14, 2020; DOI:
10.1126/sciadv.aaz9240
Procurement and treatment of human cartilage
Author:
Fadia Kamal
Date: Feb 22, 2021,Views:847
Original protocol used in research article:
Paroxetine-mediated GRK2 inhibition is a disease-modifying treatment for osteoarthritis
Science Translational Medicine, Feb 9, 2021; DOI:
10.1126/scitranslmed.aau8491
Animal studies
Author:
Fadia Kamal
Date: Feb 22, 2021,Views:867
Original protocol used in research article:
Paroxetine-mediated GRK2 inhibition is a disease-modifying treatment for osteoarthritis
Science Translational Medicine, Feb 9, 2021; DOI:
10.1126/scitranslmed.aau8491
Preparation of lymph node stromal cells
Authors:
Christian Perez-Shibayama
and
Burkhard Ludewig
Date: Feb 22, 2021,Views:779
Original protocol used in research article:
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI:
10.1126/sciimmunol.abb7066
Epidemiological, demographic and geographical data
Author:
Huaiyu Tian
Date: Feb 21, 2021,Views:944
Original protocol used in research article:
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
Science, May 7, 2020; DOI:
10.1126/science.abb6105
Human mobility data
Author:
Huaiyu Tian
Date: Feb 21, 2021,Views:963
Original protocol used in research article:
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
Science, May 7, 2020; DOI:
10.1126/science.abb6105
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