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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (420)
发育生物学 (311)
分子生物学 (1101)
干细胞 (243)
免疫学 (584)
神经科学 (676)
生物化学 (1346)
生物物理学 (156)
微生物学 (1134)
系统生物学 (322)
细胞生物学 (2063)
植物科学 (1036)
Bio-protocol 期刊
Bio-101 实验手册
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
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Protein expression and purification
Authors:
Claire Gorby
and
Ignacio Moraga
Date: Mar 11, 2021,Views:883
Original protocol used in research article:
Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses
Science Signaling, Sep 14, 2020; DOI:
10.1126/scisignal.abc0653
Automated filopodia analysis software
Author:
Johannes Stegmaier
Date: Mar 10, 2021,Views:918
Original protocol used in research article:
Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates
eLife, Jul 31, 2018; DOI:
10.7554/eLife.36953
Primary mouse EC isolation
Authors:
Nora Yucel
and
Zoltan Arany
Date: Mar 10, 2021,Views:936
Original protocol used in research article:
Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
eLife, Dec 14, 2020; DOI:
10.7554/eLife.55730
Imaging Cytosolic Calcium in T Lymphocytes: a protocol for use with genetically encoded or chemical Ca2+ indicators
Author:
Michael D Cahalan
Date: Mar 10, 2021,Views:1109
Original protocol used in research article:
T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse
eLife, Dec 14, 2017; DOI:
10.7554/eLife.32417
Petri dish hygrotaxis arena
Author:
Jennifer S Sun
Date: Mar 9, 2021,Views:844
Original protocol used in research article:
Humidity response depends on the small soluble protein Obp59a in
Drosophila
eLife, Sep 19, 2018; DOI:
10.7554/eLife.39249
Redox potential measurements
Author:
Aspen T Reese
Date: Mar 8, 2021,Views:910
Original protocol used in research article:
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI:
10.7554/eLife.35987
Recovery cohousing following antibiotic treatment
Author:
Aspen T Reese
Date: Mar 8, 2021,Views:830
Original protocol used in research article:
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI:
10.7554/eLife.35987
Construction of plasmids
Author:
Eric Klavins
Date: Mar 8, 2021,Views:934
Original protocol used in research article:
Synthetic hormone-responsive transcription factors can monitor and re-program plant development
eLife, May 1, 2018; DOI:
10.7554/eLife.34702
Protein expression and purification of the cysteine-rich domain of Frizzled receptors
Author:
Tao-Hsin Chang
Date: Mar 7, 2021,Views:1031
Original protocol used in research article:
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan
eLife, Jul 9, 2015; DOI:
10.7554/eLife.06554
Kmer mapping
Author:
Bernadette C Young
Date: Mar 7, 2021,Views:1676
Original protocol used in research article:
Panton–Valentine leucocidin is the key determinant of
Staphylococcus aureus
pyomyositis in a bacterial GWAS
eLife, Feb 22, 2019; DOI:
10.7554/eLife.42486
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