Bio-101 - Improve Research Reproducibility

Protein expression and purification

Authors: Claire Gorby and Ignacio Moraga
Date: Mar 11, 2021，Views：883

Original protocol used in research article：

Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses
Science Signaling, Sep 14, 2020; DOI: 10.1126/scisignal.abc0653

Automated filopodia analysis software

Author: Johannes Stegmaier
Date: Mar 10, 2021，Views：918

Original protocol used in research article：

Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates
eLife, Jul 31, 2018; DOI: 10.7554/eLife.36953

Primary mouse EC isolation

Authors: Nora Yucel and Zoltan Arany
Date: Mar 10, 2021，Views：936

Original protocol used in research article：

Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
eLife, Dec 14, 2020; DOI: 10.7554/eLife.55730

Imaging Cytosolic Calcium in T Lymphocytes: a protocol for use with genetically encoded or chemical Ca2+ indicators

Author: Michael D Cahalan
Date: Mar 10, 2021，Views：1109

Original protocol used in research article：

T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse
eLife, Dec 14, 2017; DOI: 10.7554/eLife.32417

Petri dish hygrotaxis arena

Author: Jennifer S Sun
Date: Mar 9, 2021，Views：844

Original protocol used in research article：

Humidity response depends on the small soluble protein Obp59a in Drosophila
eLife, Sep 19, 2018; DOI: 10.7554/eLife.39249

Redox potential measurements

Author: Aspen T Reese
Date: Mar 8, 2021，Views：910

Original protocol used in research article：

Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987

Recovery cohousing following antibiotic treatment

Author: Aspen T Reese
Date: Mar 8, 2021，Views：830

Original protocol used in research article：

Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987

Construction of plasmids

Author: Eric Klavins
Date: Mar 8, 2021，Views：934

Original protocol used in research article：

Synthetic hormone-responsive transcription factors can monitor and re-program plant development
eLife, May 1, 2018; DOI: 10.7554/eLife.34702

Protein expression and purification of the cysteine-rich domain of Frizzled receptors

Author: Tao-Hsin Chang
Date: Mar 7, 2021，Views：1031

Original protocol used in research article：

Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan
eLife, Jul 9, 2015; DOI: 10.7554/eLife.06554

Kmer mapping

Author: Bernadette C Young
Date: Mar 7, 2021，Views：1676

Original protocol used in research article：

Panton–Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS
eLife, Feb 22, 2019; DOI: 10.7554/eLife.42486

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