Bio-101 - Improve Research Reproducibility

mRNAs half-lives determination

Author: Ning Dai
Date: Feb 16, 2021，Views：847

Original protocol used in research article：

IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
eLife, Jul 28, 2017; DOI: 10.7554/eLife.27155

Protein expression, purification, and site-specific labeling

Author: Jesper Levring
Date: Feb 16, 2021，Views：974

Original protocol used in research article：

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451

Cloning of mlCNBD-FRET

Author: Dagmar Wachten
Date: Feb 16, 2021，Views：962

Original protocol used in research article：

A novel biosensor to study cAMP dynamics in cilia and flagella
eLife, Mar 22, 2016; DOI: 10.7554/eLife.14052

Muscle myoblast isolation and culture

Author: Shihuan Kuang
Date: Feb 15, 2021，Views：920

Original protocol used in research article：

Stage-specific effects of Notch activation during skeletal myogenesis
eLife, Sep 19, 2016; DOI: 10.7554/eLife.17355

Multiplex FISH in CaRMA-imaged tissue after in vivo calcium imaging

Authors: Hui Yang, Shengjin Xu and Scott M. Sternson
Date: Feb 15, 2021，Views：978

Original protocol used in research article：

Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494

Alignment of the GRIN lens

Authors: Zhongya Qin, Nancy Y. Ip and Jianan Y. Qu
Date: Feb 15, 2021，Views：978

Original protocol used in research article：

Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
Science Advances, Sep 29, 2020; DOI: 10.1126/sciadv.abc6521

HCN4 immunostaining in human (Alomone labs antibody)

Authors: Angélique Bordey and Lena Nguyen
Date: Feb 13, 2021，Views：818

Original protocol used in research article：

Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI: 10.1126/scitranslmed.abc1492

Migration assay

Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 12, 2021，Views：903

Original protocol used in research article：

Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105

Generation of in vitro trained TAMs

Authors: Poonam Ashwin Kumar, Andreas Weigert and Rajkumar Savai
Date: Feb 12, 2021，Views：922

Original protocol used in research article：

Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105

Article inclusion

Author: Alyson Haslam
Date: Feb 12, 2021，Views：1035

Original protocol used in research article：

A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183

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