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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (420)
发育生物学 (311)
分子生物学 (1101)
干细胞 (243)
免疫学 (584)
神经科学 (676)
生物化学 (1346)
生物物理学 (156)
微生物学 (1134)
系统生物学 (322)
细胞生物学 (2063)
植物科学 (1036)
Bio-protocol 期刊
Bio-101 实验手册
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
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mRNAs half-lives determination
Author:
Ning Dai
Date: Feb 16, 2021,Views:847
Original protocol used in research article:
IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
eLife, Jul 28, 2017; DOI:
10.7554/eLife.27155
Protein expression, purification, and site-specific labeling
Author:
Jesper Levring
Date: Feb 16, 2021,Views:974
Original protocol used in research article:
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI:
10.7554/eLife.56451
Cloning of mlCNBD-FRET
Author:
Dagmar Wachten
Date: Feb 16, 2021,Views:962
Original protocol used in research article:
A novel biosensor to study cAMP dynamics in cilia and flagella
eLife, Mar 22, 2016; DOI:
10.7554/eLife.14052
Muscle myoblast isolation and culture
Author:
Shihuan Kuang
Date: Feb 15, 2021,Views:920
Original protocol used in research article:
Stage-specific effects of Notch activation during skeletal myogenesis
eLife, Sep 19, 2016; DOI:
10.7554/eLife.17355
Multiplex FISH in CaRMA-imaged tissue after in vivo calcium imaging
Authors:
Hui Yang
,
Shengjin Xu
and
Scott M. Sternson
Date: Feb 15, 2021,Views:978
Original protocol used in research article:
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI:
10.1126/science.abb2494
Alignment of the GRIN lens
Authors:
Zhongya Qin
,
Nancy Y. Ip
and
Jianan Y. Qu
Date: Feb 15, 2021,Views:978
Original protocol used in research article:
Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
Science Advances, Sep 29, 2020; DOI:
10.1126/sciadv.abc6521
HCN4 immunostaining in human (Alomone labs antibody)
Authors:
Angélique Bordey
and
Lena Nguyen
Date: Feb 13, 2021,Views:818
Original protocol used in research article:
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI:
10.1126/scitranslmed.abc1492
Migration assay
Authors:
Poonam Ashwin Kumar Sarode
and
Rajkumar Savai
Date: Feb 12, 2021,Views:903
Original protocol used in research article:
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI:
10.1126/sciadv.aaz6105
Generation of in vitro trained TAMs
Authors:
Poonam Ashwin Kumar
,
Andreas Weigert
and
Rajkumar Savai
Date: Feb 12, 2021,Views:922
Original protocol used in research article:
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI:
10.1126/sciadv.aaz6105
Article inclusion
Author:
Alyson Haslam
Date: Feb 12, 2021,Views:1035
Original protocol used in research article:
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI:
10.7554/eLife.45183
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