Protocols shared by authors via RaP service
Journal
mRNAs half-lives determination
Author: Ning Dai
Date: Feb 16, 2021,Views:847
Original protocol used in research article:
IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
eLife, Jul 28, 2017; DOI: 10.7554/eLife.27155
Protein expression, purification, and site-specific labeling
Author: Jesper Levring
Date: Feb 16, 2021,Views:974
Original protocol used in research article:
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451
Cloning of mlCNBD-FRET
Author: Dagmar Wachten
Date: Feb 16, 2021,Views:962
Original protocol used in research article:
A novel biosensor to study cAMP dynamics in cilia and flagella
eLife, Mar 22, 2016; DOI: 10.7554/eLife.14052
Muscle myoblast isolation and culture
Author: Shihuan Kuang
Date: Feb 15, 2021,Views:920
Original protocol used in research article:
Stage-specific effects of Notch activation during skeletal myogenesis
eLife, Sep 19, 2016; DOI: 10.7554/eLife.17355
Multiplex FISH in CaRMA-imaged tissue after in vivo calcium imaging
Authors: Hui Yang, Shengjin Xu and Scott M. Sternson
Date: Feb 15, 2021,Views:978
Original protocol used in research article:
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494
Alignment of the GRIN lens
Authors: Zhongya Qin, Nancy Y. Ip and Jianan Y. Qu
Date: Feb 15, 2021,Views:978
Original protocol used in research article:
Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
Science Advances, Sep 29, 2020; DOI: 10.1126/sciadv.abc6521
HCN4 immunostaining in human (Alomone labs antibody)
Authors: Angélique Bordey and Lena Nguyen
Date: Feb 13, 2021,Views:818
Original protocol used in research article:
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI: 10.1126/scitranslmed.abc1492
Migration assay
Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 12, 2021,Views:903
Original protocol used in research article:
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Generation of in vitro trained TAMs
Authors: Poonam Ashwin Kumar, Andreas Weigert and Rajkumar Savai
Date: Feb 12, 2021,Views:922
Original protocol used in research article:
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Article inclusion
Author: Alyson Haslam
Date: Feb 12, 2021,Views:1035
Original protocol used in research article:
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183