Bio-101 - Improve Research Reproducibility

Starvation of cells and re-stimulation with aa’s

Author: Maria Manifava
Date: Mar 1, 2021，Views：979

Original protocol used in research article：

Dynamics of mTORC1 activation in response to amino acids
eLife, Oct 11, 2016; DOI: 10.7554/eLife.19960

Wholemount in situ hybridization for spatial-temporal visualization of gene expression in mouse early post-implantation embryos

Authors: Xianfa Yang, Yingying Chen, Lu Song, Ting Zhang and Naihe Jing
Date: Feb 27, 2021，Views：1156

Original protocol used in research article：

The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways
eLife, Jun 14, 2014; DOI: 10.7554/eLife.02224

Fluorescent cell sorting from Adult Drosophila Brains

Authors: Dingbang Ma and Michael Rosbash
Date: Feb 26, 2021，Views：1073

Original protocol used in research article：

A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056

Sample correlation matrix

Author: Richard White
Date: Feb 26, 2021，Views：958

Original protocol used in research article：

A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860

RNA-seq analysis

Authors: Laura A Lavery, Ying-Wooi Wan and Alexander Trostle
Date: Feb 26, 2021，Views：930

Original protocol used in research article：

Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981

Isolation of thymic epithelial cells and thymocytes

Authors: Chris P Ponting and Georg A Holländer
Date: Feb 26, 2021，Views：1675

Original protocol used in research article：

Ageing compromises mouse thymus function and remodels epithelial cell differentiation
eLife, Aug 25, 2020; DOI: 10.7554/eLife.56221

Gene construction

Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：926

Original protocol used in research article：

A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241

Antibody sequences

Authors: Yan Wu, Feng Gao and George F. Gao
Date: Feb 25, 2021，Views：962

Original protocol used in research article：

A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241

Cells and Viruses

Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1065

Original protocol used in research article：

A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241

Protein expression and purification

Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1536

Original protocol used in research article：

A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241

实验方法 

Request a Protocol

提交 

关于