Protocols shared by authors via RaP service
Journal
Starvation of cells and re-stimulation with aa’s
Author: Maria Manifava
Date: Mar 1, 2021,Views:979
Original protocol used in research article:
Dynamics of mTORC1 activation in response to amino acids
eLife, Oct 11, 2016; DOI: 10.7554/eLife.19960
Wholemount in situ hybridization for spatial-temporal visualization of gene expression in mouse early post-implantation embryos
Authors: Xianfa Yang, Yingying Chen, Lu Song, Ting Zhang and Naihe Jing
Date: Feb 27, 2021,Views:1156
Original protocol used in research article:
The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways
eLife, Jun 14, 2014; DOI: 10.7554/eLife.02224
Fluorescent cell sorting from Adult Drosophila Brains
Authors: Dingbang Ma and Michael Rosbash
Date: Feb 26, 2021,Views:1073
Original protocol used in research article:
A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056
Sample correlation matrix
Author: Richard White
Date: Feb 26, 2021,Views:958
Original protocol used in research article:
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
RNA-seq analysis
Authors: Laura A Lavery, Ying-Wooi Wan and Alexander Trostle
Date: Feb 26, 2021,Views:929
Original protocol used in research article:
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Isolation of thymic epithelial cells and thymocytes
Authors: Chris P Ponting and Georg A Holländer
Date: Feb 26, 2021,Views:1675
Original protocol used in research article:
Ageing compromises mouse thymus function and remodels epithelial cell differentiation
eLife, Aug 25, 2020; DOI: 10.7554/eLife.56221
Gene construction
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021,Views:926
Original protocol used in research article:
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Antibody sequences
Authors: Yan Wu, Feng Gao and George F. Gao
Date: Feb 25, 2021,Views:962
Original protocol used in research article:
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Cells and Viruses
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021,Views:1065
Original protocol used in research article:
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Protein expression and purification
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021,Views:1536
Original protocol used in research article:
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241