Bio-101 - Improve Research Reproducibility

Oil-Red-O staining to determine the amount of stored triglyceride in Caenorhabditis elegans

Authors: Avijit Mallick and Bhagwati P Gupta
Date: Mar 15, 2021，Views：832

Original protocol used in research article：

Vitellogenin-2 acts downstream of PRY-1/Axin to regulate lipids and lifespan in C. elegans
microPublication Biology, Jul 21, 2020; DOI: 10.17912/micropub.biology.000281

Calculation of VISTA module score

Author: Yanding Zhao
Date: Mar 15, 2021，Views：823

Original protocol used in research article：

VISTA is a checkpoint regulator for na\xefve T cell quiescence and peripheral tolerance
Science, Jan 16, 2020; DOI: 10.1126/science.aay0524

Virus neutralization assay

Authors: Yasumasa Iwatani and Hiroshi Murakami
Date: Mar 15, 2021，Views：959

Original protocol used in research article：

Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916

Computational methods

Authors: Lizhi Liu and Sheng Chen
Date: Mar 15, 2021，Views：817

Original protocol used in research article：

Mechanical penetration of β-lactam–resistant Gram-negative bacteria by programmable nanowires
Science Advances, Jul 2, 2020; DOI: 10.1126/sciadv.abb9593

Headloop PCR

Author: Gareth Thomas Powell
Date: Mar 15, 2021，Views：2257

Original protocol used in research article：

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683

ChIP-seq

Author: Wen Tang
Date: Mar 15, 2021，Views：933

Original protocol used in research article：

ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesin^STAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091

De novo protein design

Author: Anastassia A. Vorobieva
Date: Mar 15, 2021，Views：885

Original protocol used in research article：

De novo design of transmembrane \β barrels
Science, Feb 18, 2021; DOI: 10.1126/science.abc8182

Organoid experiments culture & seeding

Authors: Wouter R. Karthaus, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：995

Original protocol used in research article：

Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267

Prostate isolation and enzymatic digestion

Authors: Wouter R. Karthaus, Matan Hofree, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：967

Original protocol used in research article：

Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267

Single-cell dissociation and RNA sequencing

Author: Michael L Wallace
Date: Mar 11, 2021，Views：858

Original protocol used in research article：

Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271

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