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A high-quality database for basic life science protocols
实验方法
研究领域
癌症生物学 (420)
发育生物学 (311)
分子生物学 (1101)
干细胞 (243)
免疫学 (584)
神经科学 (676)
生物化学 (1346)
生物物理学 (156)
微生物学 (1134)
系统生物学 (322)
细胞生物学 (2063)
植物科学 (1036)
Bio-protocol 期刊
Bio-101 实验手册
课题组实验方法合辑
Request a Protocol
提交
提交实验方法
作者指南
关于
Protocols shared by authors via RaP service
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Particle tracking
Author:
Masazumi Fujiwara
Date: Feb 25, 2021,Views:816
Original protocol used in research article:
Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI:
10.1126/sciadv.aba9636
ZFA enrichment
Author:
Richard J White
Date: Feb 25, 2021,Views:997
Original protocol used in research article:
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI:
10.7554/eLife.30860
Plasmid constructs
Author:
Theodore S. Jardetzky
Date: Feb 24, 2021,Views:878
Original protocol used in research article:
EphrinB2 clustering by Nipah virus G is required to activate and trap F intermediates at supported lipid bilayer–cell interfaces
Science Advances, Jan 27, 2021; DOI:
10.1126/sciadv.abe1235
List of antibodies, siRNAs and shRNAs
Author:
Seyed Mehdi Jafarnejad
Date: Feb 24, 2021,Views:852
Original protocol used in research article:
Translational control of ERK signaling through miRNA/4EHP-directed silencing
eLife, Feb 7, 2018; DOI:
10.7554/eLife.35034
RNA extraction from individual zebrafish embryos
Authors: Neha Wali,
Richard White
and
Elisabeth M Busch-Nentwich
Date: Feb 24, 2021,Views:1192
Original protocol used in research article:
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI:
10.7554/eLife.30860
Cell culture, infection and vitrification
Authors:
Georg Wolff
and
Montserrat Bárcena
Date: Feb 24, 2021,Views:1114
Original protocol used in research article:
A molecular pore spans the double membrane of the coronavirus replication organelle
Science, Aug 6, 2020; DOI:
10.1126/science.abd3629
Measurement of thickness of the inner mucus layer
Authors:
Takahide Ara
,
Daigo Hashimoto
and
Takanori Teshima
Date: Feb 24, 2021,Views:903
Original protocol used in research article:
Intestinal goblet cells protect against GVHD after allogeneic stem cell transplantation via Lypd8
Science Translational Medicine, Jun 30, 2020; DOI:
10.1126/scitranslmed.aaw0720
Task implementations
Author:
Jin Fan
Date: Feb 24, 2021,Views:894
Original protocol used in research article:
Anterior insular cortex plays a critical role in interoceptive attention
eLife, Apr 15, 2019; DOI:
10.7554/eLife.42265
Detection of proteins in membrane fractions
Authors:
Alam García-Heredia
,
Takehiro Kado
,
Caralyn E. Sein
,
Julia Puffal
,
Sarah H. Osman
,
Julius Judd
,
Todd A. Gray
,
Yasu S. Morita
and
M. Sloan Siegrist
Date: Feb 23, 2021,Views:913
Original protocol used in research article:
Membrane-partitioned cell wall synthesis in mycobacteria
eLife, Feb 5, 2021; DOI:
10.7554/eLife.60263
Protocol of full article
Author:
Kenneth Trimmer
Date: Feb 23, 2021,Views:775
Original protocol used in research article:
CRISPR-edit point mutant allele detection (CEPAD)-PCR method for rapid screening of CRISPR edited point mutations
microPublication Biology, Jan 1, 1970; DOI:
10.17912/micropub.biology.000368
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