Protocols shared by authors via RaP service
Journal
In vitro co-culture and 3D matrigel/collagen culture assays
Author: Don L. Gibbons
Date: Jun 15, 2021,Views:808
Original protocol used in research article:
Collagen promotes anti-PD-1/PD-L1 resistance in cancer through LAIR1-dependent CD8+ T cell exhaustion
Nature Communications, Sep 9, 2020; DOI: 10.1038/s41467-020-18298-8
In vitro microtubule-binding assays
Authors: Jianguo Chen and Junlin Teng
Date: Jun 12, 2021,Views:813
Original protocol used in research article:
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment
BMC Biology, Sep 14, 2019; DOI: 10.1186/s12915-019-0694-9
Complex I Enzymatic Activity Assay
Author: Shaobai Huang
Date: Jun 11, 2021,Views:794
Original protocol used in research article:
Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes1,[OPEN]
Plant Physiology, Jun 22, 2020; DOI: 10.1104/pp.20.00136
LacZ activity measurement.
Authors: Zining Wang and Xiaojun Xia
Date: Jun 10, 2021,Views:821
Original protocol used in research article:
cGAS/STING axis mediates a topoisomerase II inhibitor–induced tumor immunogenicity
The Journal of Clinical Investigation, Oct 7, 2019; DOI: 10.1172/JCI127471
Quantification of brain infarct volume
Authors: Weidong Liang and Chunshui Lin
Date: Jun 10, 2021,Views:834
Original protocol used in research article:
Preactivation of Notch1 in remote ischemic preconditioning reduces cerebral ischemia-reperfusion injury through crosstalk with the NF-κB pathway
Journal of Neuroinflammation, Sep 16, 2019; DOI: 10.1186/s12974-019-1570-9
Optic fiber implantation
Authors: Mehran Ahmadlou and J. Alexander Heimel
Date: Jun 9, 2021,Views:841
Original protocol used in research article:
A cell type–specific cortico-subcortical brain circuit for investigatory and novelty-seeking behavior
Science, May 14, 2021; DOI: 10.1126/science.abe9681
In vitro immunoregulation of osteogenesis of MSCs
Authors: Xiangmei Liu, Cao Yang and Kelvin W. K. Yeung
Date: Jun 9, 2021,Views:838
Original protocol used in research article:
Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration
Science Advances, Apr 1, 2021; DOI: 10.1126/sciadv.abf6654
Label-free quantitative proteomic analysis of enriched phosphopeptides from dark- and light-adapted bovine retinas
Author: Ana Méndez
Date: Jun 8, 2021,Views:837
Original protocol used in research article:
Post-translational regulation of retinal IMPDH1 in vivo to adjust GTP synthesis to illumination conditions
eLife, Apr 7, 2020; DOI: 10.7554/eLife.56418
Machine learning regression
Authors: Xiangang Hu and Fubo Yu
Date: Jun 8, 2021,Views:975
Original protocol used in research article:
Deep exploration of random forest model boosts the interpretability of machine learning studies of complicated immune responses and lung burden of nanoparticles
Science Advances, May 26, 2021; DOI: 10.1126/sciadv.abf4130
In situ metabolic labeling
Author: Ana Méndez
Date: Jun 8, 2021,Views:817
Original protocol used in research article:
Post-translational regulation of retinal IMPDH1 in vivo to adjust GTP synthesis to illumination conditions
eLife, Apr 7, 2020; DOI: 10.7554/eLife.56418